Just-a-Tube ^™ or Just-a-Plate ^™

Virus RNA/DNA Isolation Kit

The Just-a-Tube^™ or Just-a-Plate^™ 96 Virus RNA/DNA Isolation Kit is designed and optimized for highly sensitive, easy, fast and high-throughput isolation of high-quality virus nucleic acid from cell-free biological fluids such as plasma, serum, and CSF (cerebrospinal fluid) as well as cell culture supernatant. No silica membrane, filter plate, or magnetic beads are needed and no phenol, chloroform, or other organic solvents are required in the process.  Based on charm biotech developed Solid Surface Reversible Binding (SSRB) technology (patent pending), Just-a-Tube/Just-a-plate system employs the solid micro-tube or 96-well plate surface coated with proprietary turbo-binders as selective nucleic acid capturers to efficiently extract virus RNA and DNA from cell-free liquid samples. Virus nucleic acid molecules can specifically interact with the turbo binder in the extraction buffer, while proteins and other contaminants are not bound and remain in the solution. After washing the plate with washing buffer to remove unbound material, the purified viral RNA and DNA can be either easily eluted in 10 mM Tris Elution Buffer or water, or used directly for viral detection and quantification, such as qPCR on the same tube/plate without elution The novel Just-a-Plate kit eliminates usage of carrier RNA for viral nucleic acid extraction, which is usually used in the conventional silica membrane absorption methods, making downstream detection and applications completely without interferences associated with co-purified carrier RNA. Since no silica membrane and beads have been used in the tube/plate, there are no dead spaces and sample trapping problems related with membrane or bead-based column or plate methods. These features not only allow fast buffer washing and easy sample elution, but also provide the maximum sample recovery, which is extremely important when the sample amount is limit and there is no room for any sample loss, such as serum or plasma sample with ultra-low-titer virus.

Feature Highlight

• Fast to perform: It only takes 30 minutes of overall time to purify 96 cell-free viral samples. No tedious vacuum “ drying” process as being used for 96-well plates with membrane.
• Easy to handle:  All procedures have been optimized with standard microtube/96-well PCR plates for ease-of-use. Elimination of sample elution for detection makes whole procedure very easy to perform. No extra carrier RNA and no vacuum or magnetic force needed.
• Reliable quality: Consistent tube-to-tube/well-to-well and plate-to-plate performance, reliable cross-contamination prevention procedure and unique solid-surface capturing technology ensure high reproducibility and consistent yield of viral nucleic acid at high quality.
• Maximum recovery: Novel efficient binding technology provides maximum sample capture and release from limited sample sources without dead space or sample trapping problems usually associated with the silica membrane and bead based absorption methods.  It translates higher sensitivity over conventional methods.
• High sensitivity and linearity with great reproducibility:  Extraction is both sensitive and linear down to the limit of 1 RNA virus particles and 10 DNA virus particles using the Just-a-Tube/Just-a-Plate 96 viral RNA/DNA system.

Order Information

Note: Individual kit components can be purchased separately, Please call for details.