Charm-Pure ^™ FFPE MicroRNA/Total RNA Isolation Kit

The Charm-Pure^™ 96 FFPE MicroRNA/Total RNA Isolation Kit is designed and optimized for easy and fast isolation of high-quality RNA from FFPE tissues. Charm-Pure system employs special turbo-binding membranes as selective nucleic acid captures in a familiar column format to efficiently extract RNA from FFPE sample tissues with higher yield and purity than conventional silica membrane. RNA molecules can specifically interact with the turbo-binding membrane in the extraction buffer, while proteins and other contaminants are not bound and remain in the solution and passed through. After washing the column with washing buffer to remove unbound material, the purified total RNA and microRNA can be easily eluted in 10 mM Tris Elution Buffer or water. The novel sample buffers and process in this kit can also efficiently remove nucleic acid chemical modifications introduced by formalin fixation step in tissues embedding process. The modification-removed RNA molecules are better substrates being ready for use in downstream applications, such as reverse transcription, microRNA detection, quantitative RT-PCR (qRT-PCR) and other gene expression analysis.

Feature Highlight

• Fast to perform:  After tissue section, it only takes 10-15 minutes of hand-on time to purify FFPE tissue samples. No tedious chemical deparaffin process required in the purification process.
• Easy to handle: Both de-paraffin and RNA isolation will be processed in the streamlined protocol. No need to look for pellet in the regular chemical de-paraffin method..
• Reliable quality: Consistent turbo-binding membrane super capturing performance and unique formamide cross-linking removal chemistry provide better yield  and higher quality than conventional silica membrane technology
• Technical data:  Process 1 to 10 slices of 10 um FFPE tissue section, up to 10-15 ug RNA can be purified based on the sample types.

Order Information

Note: Individual kit components can be purchased separately, Please call for details.